Kohler determined that insight Video
Insight Learning: the Early Work of Wolfgang Köhler kohler determined that insight
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Original Research ARTICLE
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Find more information on the Altmetric Attention Score and how the score is calculated. We explore the potential of orientation-resolved pulsed dipolar spectroscopy PDS in light-induced versions of the experiment. The use of triplets as spin-active moieties for PDS offers an attractive tool for studying biochemical systems containing optically active cofactors.
INTRODUCTION
Cofactors are often rigidly bound within the protein structure, providing an accurate positional marker. The rigidity leads to link selection effects in PDS, which can be analyzed kohler determined that insight give both distance and mutual orientation information.
Herein we present a comprehensive analysis of the orientation selection of a full set of light-induced PDS experiments. We exploit the complementary information provided by the different light-induced techniques to yield atomic-level structural information.
For the first time, we measure a 2D frequency-correlated laser-induced magnetic dipolar spectrum, and we are able to monitor the complete orientation dependence of the system in a single experiment. Alternatively, the summed spectrum enables an orientation-independent analysis to determine the distance distribution. kohlre
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Figure 1. Figure 2.
The modulation depth has been normalized to 1 for ease of analysis. The diameter of the sphere is proportional to the number of times a single porphyrin position contributes to the complete fit shown in panel b. The experimental conditions and the parameters of the data analysis are reported in the Supporting Information. Figure 3.]
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