Substrate Concentration And Enzyme Activity

Substrate concentration and enzyme activity Video

Enzymes - Effect of Substrate Concentration substrate concentration and enzyme activity

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Effect of temperature, substrate concentration and pH on reaction rate The rate of an enzyme-catalysed reaction is calculated here measuring the rate at which a substrate ahd used up or by the rate at which a product is formed. However, at high temperatures the rate decreases again because the enzyme becomes denatured and can no longer function.

This is shown in the graph below. As the temperature increases so does the rate of enzyme activity. An optimum activity is reached at the enzyme's optimum temperature. A continued increase in temperature results in a sharp decrease in activity as the enzyme's active site changes shape. It is now denatured. Each enzyme work bests at a specific pH value. The optimum pH for an enzyme depends on where it normally works. For example, enzymes in the small intestine have an optimum pH of about 7.

Does the shape of the enzyme change at all when binding with the substrate?

In the graph above, as the pH increases so does the rate of enzyme activity. Substrate concentration Enzymes will work best if there is plenty of substrate. As the concentration of the substrate increases, so does the rate of enzyme activity.

However, the rate of enzyme activity does not increase forever. This is because a point will be reached when the enzymes become saturated and no more substrates can fit at any one time even though there is plenty of substrate available. As the substrate concentration increases so does the rate of enzyme activity. A continued increase in substrate concentration results subetrate the same activity as there are not enough enzyme molecules available to break down the excess substrate molecules.]