Characteristics of plasma membrane Video
Inside the Cell Membrane characteristics of plasma membrane
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These inserts are made of rigid materials and used under static culture conditions, which are unrepresentative of biological microenvironments. Here, we present FleXert, a soft, actuatable cell culture insert that interfaces with six-well plates.
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A wide range of actuation patterns can be achieved by varying the air pressure and pumping rate. In contrast to existing technologies, FleXerts do not require costly fabrication equipment or custom-built culture chambers, making them a versatile and chatacteristics solution for tissue engineering and biological barrier penetration studies under physiological strain.
This paper is an extensive toolkit for multidisciplinary mechanobiology studies, including detailed instructions for a wide variety of methods such as device fabrication, theoretical modeling, cell culture, and image analysis techniques. Made available for a limited time for personal research and study only License. Figure 1.
1. Introduction
FleXert design principle and mold fabrication. The bottom piece has a central opening instead of the membrane.
Both pieces have a circular groove that forms an enclosed air charactteristics when they are bonded together. The mold is composed of a bottom part, onto which acrylic rings are glued to pattern the grooves that make up the air channel. The two middle parts of the mold form the outside of the FleXert pieces.
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Fabricate one set of these mold parts to make top pieces and another set to make the bottom pieces. It needs to be determined experimentally by weighing and kept consistent when fabricating different batches. Stereolithography printer e. Hand drill and drill head, 3. Computer-aided design CAD package e. Use a CAD package, such as Autodesk Inventorto draw designs for acrylic and stereolithography printed mold parts.
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FleXert diameter, air channel dimensions, and wall thicknesses can be tuned in this step. Refer to Figure 1 b for all FleXert dimensions used in this paper. For the stereolithography printed parts, design cylinders with and without pillar arrays.]
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